Production of 15-, 16- and 17-hydroxy-9-octadecenoic acids by bioconversion of oleic acid with Bacillus pumilus

Several Bacillus strains were tested for their ability to hydroxylate oleic acid. Two strains—BD‐174 and BD‐226—converted oleic acid into a trio of hydroxy‐octadecenoic acids. Bioconversion in 72–120 hr produced 5–11% of hydroxy acids relative to oleic acid as measured by gas chromatography. These a...

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Published inJournal of the American Oil Chemists' Society Vol. 69; no. 4; pp. 363 - 366
Main Authors Lanser, A.C. (National Center for Agricultural Utilization Research, USDA, ARS, Peoria, IL), Plattner, R.D, Bagby, M.O
Format Journal Article
LanguageEnglish
Published 01.04.1992
Subjects
ACIDE GRAS
ACIDE OLEIQUE
ACIDO OLEICO
ACIDOS GRASOS
BACILLUS
BACILLUS MEGATERIUM
BIOLOGICAL PRODUCTION
CHEMICAL REACTIONS
FATTY ACIDS
HYDROXY FATTY ACIDS
HYDROXYLATION
OLEIC ACID
REACCIONES QUIMICAS
REACTION CHIMIQUE
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Summary:Several Bacillus strains were tested for their ability to hydroxylate oleic acid. Two strains—BD‐174 and BD‐226—converted oleic acid into a trio of hydroxy‐octadecenoic acids. Bioconversion in 72–120 hr produced 5–11% of hydroxy acids relative to oleic acid as measured by gas chromatography. These acids were identified as the 15‐, 16‐ and 17‐hydroxy‐9‐octadecenoic acids by gas chromatography‐mass spectrometry of trimethyl silyl derivatives of the product acids and their hydrogenated counterparts.
Bibliography:9189951
F60
ISSN:0003-021X
1558-9331
DOI:10.1007/BF02636069