Algoriphagus algorifonticola sp. nov., a marine bacterium isolated from cold spring area of South China Sea

• Published in: International journal of systematic and evolutionary microbiology Vol. 72; no. 5
• Main Authors: Yan, Cen, Chen, Can, Chai, Baozhong, Ye, Yanghui, Anwar, Nusratgul, Zhao, Zhe, Wang, Ruijun, Huo, Yingyi, Zhang, Xinyin, Wu, Min, Zheng, Daoqiong
• Format: Journal Article
• Language: English
• Published: England 01.05.2022
• Subjects: marine bacterium; Algoriphagus algorifonticola sp. nov; polyphasic taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.005365

A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated strain hg1 , was isolated from marine sediment within the cold spring area of South China Sea and subjected to a polyphasic taxonomic investigation. Colonies were circular and 1.0-2.0 mm in diameter, coral in colour, convex and smooth after growth on marine agar at 28 °C for 3 days. Strain hg1 was found to grow at 4-40 °C (optimum, 35-37 °C), at pH 6.5-9.0 (optimum, pH 7.5) and with 0-8 % (w/v) NaCl (optimum, 1.5-2 %). Chemotaxonomic analysis showed the sole respiratory quinone was MK-7, and the principal fatty acids are iso-C , summed feature 3 (C  ω7 and/or C  ω6 ), and iso-C . The major polar lipids are phosphatidylethanolamine, an unidentified phospholipid and five unidentified glycolipids. The DNA G+C content of strain hg1 was 39.6 mol% based on the genome sequence. The comparison of 16S rRNA gene sequence similarities showed that hg1 was closely related to DSM 15282 (98.6 % sequence similarity), MCCC 1F01099 (97.9 %) and DSM 17529 (97.2 %); it exhibited 97.0 % or less sequence similarity to the type strains of other species of the genus with validly published names. Phylogenetic trees reconstructed with the neighbour-joining, maximum-parsimony and maximum-likelihood methods based on 16S rRNA gene sequences showed that strain hg1 constituted a separate branch with . , , in a clade of the genus . OrthoANI values between strain hg1 and . , and were 94.3, 74.1, 73.2 %, respectively, and DNA-DNA hybridization values were 56.2, 18.5 and 18.3 %, respectively. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain hg1 is clearly distinct from recognized species of genus . On the basis of these features, we propose that strain hg1 (=MCCC 1K03570 =KCTC 72111 ) represents a novel species of the genus with the name sp. nov.