Effect of agro-industrial by-products on inflammation and oxidative stress using an in vitro cell model

• Published in: Archiva zootechnica Vol. 25; no. 2; pp. 97 - 106
• Main Authors: Marin, Daniela Eliza, Bulgaru, Cristina Valeria, Pistol, Gina Cecilia
• Format: Journal Article
• Language: English
• Published: Balotesti Sciendo 01.12.2022; National Research Development Institute for Animal Biology and Nutrition
• Subjects: Antioxidants; Bioactive compounds; By products; Cell culture; Cell cycle; Cell growth; Cytokines; Fatty acids; Feeds; grape seed meal; Inflammation; Leukocytes (mononuclear); Lipopolysaccharides; Meals; Nitric oxide; Nutrition; Oil and gas industry; Oxidants; Oxidative stress; Peripheral blood mononuclear cells; Petroleum industry; Polyphenols; porcine blood mononuclear cells; Raw materials; sea buckthorn meal; Seed meal; Seeds; swine
• ISSN: 2344-4592; 1016-4855; 2344-4592
• DOI: 10.2478/azibna-2022-0017

Agro-industrial by-products can play an important role in the animal feeding, being an alternative to conventional raw materials. These two wastes can be considered as important sources of bioactive compounds that can be used in animal feed with multiple benefits for animal health. This study has investigated the anti-inflammatory and anti-oxidant in vitro capacity of grape seed and sea buckthorn meals, as waste resulted from the oil industry in order to be used in swine nutrition. The study was realised on swine peripheral blood mononuclear cells challenged with LPS and treated or not with grape seed meal and sea buckthorn meal extracts. Our results show that both extracts have the capacity to attenuate the inflammation and oxidative stress induced by LPS. The effect induced by the grape seed meal extract was in majority of cases more important than of the sea buckthorn, this effect might be due to a different composition in bioactive compounds (polyphenols, PUFA etc) of the two extracts.