Morphological evaluation of the quality of decellularized esophagus in rat

The main aim of our investigation was analysis and optimization of the existing protocols of esophagus decellularization in rats, identification of parameters affecting the quality of decellularization and intactness of the native properties of esophageal extracellular matrix (ECM). Materials and me...

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Published inVestnik transplantologii i iskusstvennykh organov Vol. 19; no. 2; pp. 98 - 103
Main Authors R. Z. Nakokhov, E. A. Gubareva, E. V. Kuevda, A. S. Sotnichenko, I. S. Gumenyuk, A. Kh. Kade, G. M. Mogilnaya, D. P. Puzanov
Format Journal Article
LanguageEnglish
Russian
Published Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 23.06.2017
Subjects
decellularization
esophagus
extracellular matrix
regenerative medicine
scaffold
tissue-engineered construction
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Summary:The main aim of our investigation was analysis and optimization of the existing protocols of esophagus decellularization in rats, identification of parameters affecting the quality of decellularization and intactness of the native properties of esophageal extracellular matrix (ECM). Materials and methods. We developed a modified decellularization protocol based on detergent-enzymatic method using sodium deoxycholate and DNAse. Results. Morphological evaluation of the obtained decellularized matrices demonstrated the absence of cells and cell elements while mechanical properties of ECM were preserved. Conclusion. The developed protocol of esophagus decellularization is a potential basis for obtaining of tissue-engineered esophagus scaffold constructions.
ISSN:1995-1191
DOI:10.15825/1995-1191-2017-2-98-103