Essential oils from Cuminum cyminum and Laurus nobilis and their principal constituents: evaluation of antifungal and antimycotoxigenic potential in Aspergillus species

• Published in: FEMS microbiology letters Vol. 371
• Main Authors: Campolina, Gabriela Aguiar, Cardoso, Maria das Graças, Freire, Carolina Salles, Caetano, Alex Rodrigues Silva, Campos, Anna Beatriz de Souza, Ferreira, Vanuzia Rodrigues Fernandes, Alves, Eduardo, Nelson, David Lee, Batista, Luis Roberto
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 09.01.2024
• Subjects: fungal control; natural products; cumin; ochratoxin A; essential oil; bay leaf
• ISSN: 1574-6968; 1574-6968
• DOI: 10.1093/femsle/fnae081

Abstract The antifungal and antimycotoxigenic activities of the essential oils (EO) from Cuminum cyminum and Laurus nobilis, and their respective principal compounds, cuminaldehyde and 1,8-cineole, were evaluated against fungi of the genus Aspergillus: A. carbonarius, A. niger, A. ochraceus, and A. westerdijkiae. The antifungal activity was determined by the contact method and the mycelial growth of the fungi was evaluated. Scanning electron microscopic (SEM) images were obtained to suggest modes of action of the compounds analysed. The antimycotoxigenic activity was determined by high-performance liquid chromatograph. Aspergillus carbonarius was completely inhibited by cumin EO (500 µl l−1), by laurel EO and by cuminaldehyde (5000 µl l−1). The cumin EO (500 µl l−1) completely inhibited the growth of A. niger. All the samples inhibited the mycelial growth of A. ochraceus, especially cumin EO and cuminaldehyde (250 µl l−1). Aspergillus westerdijkiae was completely inhibited by cumin EO and cuminaldehyde (1000 µl l−1), by laurel EO and 1,8-cineole (10 000 µl l−1). A decrease in the production of ochratoxin A (OTA) was observed post-treatment, except in A. ochraceus, only inhibited by laurel EO. SEM images showed morphological changes in fungal structures and spore inhibition post-treatment. The results confirmed the antifungal and antimycotoxigenic effect of EO and their principal constituents on fungi evaluated. Effect of essential oils and standard compounds on mycotoxigenic fungi.