Study on identification of Sarcandra glabra and Chloranthus spicatus's leaves by PCR amplification of specific alleles

• Published in: Zhongguo zhongyao zazhi Vol. 39; no. 17; p. 3259
• Main Authors: Wei, Yi-cong, Chen, Ying, Luo, Lin-quan, Yang, Qun-xiong, Chen, Yi-Juan, Liang, Yi-chi, Chen, Su-Rong
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2014
• Subjects: DNA, Plant - analysis; DNA, Plant - genetics; DNA, Ribosomal - genetics; DNA, Ribosomal Spacer - analysis; DNA, Ribosomal Spacer - genetics; Magnoliopsida - classification; Magnoliopsida - genetics; Plant Leaves - genetics; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; RNA, Ribosomal - genetics; RNA, Ribosomal, 18S - genetics; RNA, Ribosomal, 5.8S - genetics; Species Specificity
• ISSN: 1001-5302
• DOI: 10.4268/cjcmm20141709

The paper is aimed to identify SNP in Sarcandra glabra and Chloranthus spicatus, and authenticate S. glabra from Ch. spicatus and the mixture by using PCR amplification of specific alleles. SNPs in the ITS sequences of S. glabra and Ch. spicatus were found by ClustulX 2. 1 program and Bioedit software. Primers for authentic S. glabra and Ch. spicatus was designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system. Five hundred and eighty band special for S. glabra and 470 bp band special for Ch. spicatus were found by using multi-PCR reaction. The multi-PCR reaction system could be applied to identify S. glabra and Ch. spicatus's leaves.