Simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum by HPLC-ELSD

To establish a HPLC-ELSD method for simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum. A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with the mobile phase consisting of acetonitrile-10 mmol x L(-1) ammonium acetate (52: 48). The temperature was...

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Bibliographic Details
Published inZhongguo zhongyao zazhi Vol. 37; no. 3; p. 323
Main Authors Jia, Yurong, Sun, Changshan, Sun, Yanxiu, Yang, Wenwen, Sun, Lixin
Format Journal Article
LanguageChinese
Published China 01.02.2012
Subjects
Chromatography, High Pressure Liquid
Drug Stability
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - chemistry
Quality Control
Reproducibility of Results
Scattering, Radiation
Solanum - chemistry
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Summary:To establish a HPLC-ELSD method for simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum. A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with the mobile phase consisting of acetonitrile-10 mmol x L(-1) ammonium acetate (52: 48). The temperature was 25 degrees C, the flow rate was set at 0.6 mL x min(-1), and the sample size is 20 microL. The temperature of drift tubes and gas flow rate of the detector were set at 95 degrees C and 2.3 L x min(-1), respectively. With in the linear ranges of 20-200 mg x L(-1) and 10-100 mg x L(-1), trillin and desgalactotigonin show a good linear relationship. The average recovery was 99.4% (RSD 0.90%) for trillin and 100.3% (RSD 1.1%) for desgalactotigonin. The method is so accurate and easily reproducible that it is suitable for the quality control of S. lyratum medicinal materials.
ISSN:1001-5302
DOI:10.4268/cjcmm20120314