Multi‐Functional Gelatin‐Dithiolane Hydrogels for Tissue Engineering

• Published in: Advanced functional materials
• Main Authors: Asim, Saad, Tuftee, Cody, Qureshi, Asma Talib, Callaghan, Rachel, Geary, Moira L., Santra, Mithun, Pal, Vaibhav, Namli, Ilayda, Yam, Gary Hin‐Fai, Ozbolat, Ibrahim T., Rizwan, Muhammad
• Format: Journal Article
• Language: English
• Published: 27.09.2024
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.202407522

Abstract Biomaterials that integrate multiple functionalities, mimic the extracellular matrix (ECM) microenvironment to support cellular growth, and adhere robustly to damaged tissues are highly needed to advance tissue engineering. Protein‐based biomaterials are promising due to their inherent biocompatibility, biomimicry, biodegradation, and cell‐supportive properties. Herein, by leveraging the unique ability of dithiolanes to generate on‐demand in situ thiols, a new class of dithiolane‐modified, protein‐based biomaterial that combines unique, seemingly opposing functions for tissue engineering is developed. Dithiolane‐modified gelatin, a model protein used herein, enabled photoinitiator‐free photo‐crosslinking to form multi‐functional gelatin‐dithiolane (GelDT) hydrogels, which displayed exceptional long‐term stability in cell culture media (>28 days) to support the growth of both surface‐seeded and encapsulated cells. GelDT hydrogels allowed pre‐gelation tuning of biomechanical properties and biodegradation via introducing physical crosslinks, and post‐gelation tuning of matrix stress‐relaxation rate, via responding to exogenous thiols, independently of other parameters. Furthermore, GelDT enabled covalent immobilization of bio‐active molecules, glutathione‐responsive drug release, supported efficient 3D bioprinting due to its shear‐thinning ability, and demonstrated robust tissue adhesion in various contexts (bare skin, ex‐vivo, in‐vivo) due to covalent disulfide coupling with endogenous tissue thiols. Together, this study presents a novel multi‐responsive and multi‐functional protein‐based biomaterial, anticipated to advance tissue engineering and regeneration.