Assessment of morphogenetic diversity in Garcinia gummi-gutta (L.) Roxb. using species-specific morphological and SSR markers

• Published in: Ecological genetics and genomics Vol. 18; p. 100081
• Main Authors: Babu, Vishnu, Ramachandran, Remya, Gangaprasad, A., Siril, E.A.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.02.2021
• Subjects: Garcinia gummi-gutta; Hydroxy citric acid; Morphometric traits; Polymorphism; SSR; Garcinia gummi-gutta; Hydroxy citric acid; Morphometric traits; SSR; Polymorphism
• ISSN: 2405-9854; 2405-9854
• DOI: 10.1016/j.egg.2021.100081

Garcinia gummi-gutta is an important tree crop valued for its dried fruit rinds as a souring agent in various dishes and source of hydroxycitric acid (HCA), an anti-obesity agent. In this study, the genetic variation among 35 accessions of G. gummi-gutta was analyzed by employing quantitative morphological traits and species-specific SSR markers. Recorded quantitative morphological data were statistically significant (p < 0.001) based on One way ANOVA. Considerable diversity observed among the accessions in growth and yield characters like the total number of fruits/tree, the mean number of fruits needed to weigh 1 kg, mean fresh and dry rind yield/tree; morphometric trait like single fruit weight. Molecular profiling was done by screening 24 simple sequence repeats (SSRs) primer from a set of 32 primers based on precise, intense and reproducible banding pattern. Total 39 alleles were obtained in the 24 SSRs with an average of 1.63 alleles/locus with an average of 86.11% polymorphism. An average Polymorphic Information Content (PIC) of 0.239 and an average Marker Index of 0.005, revealing the less allelic hetrozygosity and specificity of SSR primers for the selected accessions. The two dendrograms prepared based on Unweighted pair group method of arithmetic means (UPGMA), and Jaccard's similarity coefficient showed wide genetic variation among the accessions. Quality and productivity of G. gummi-gutta can be improved by selecting the better performing and diverse accession for future breeding programmes.