UCAO (UNILATERAL CEREBRAL ARTERY OCCLUSSION) METHOD INCREASES THE LEVEL OF MMP- 9 BRAIN TISSUE IN RATS MODEL OF ISCHEMIC STROKE

• Published in: MNJ (Malang Neurology Journal) Vol. 2; no. 2; pp. 46 - 50
• Main Authors: Rasjad Indra, M., Parindra Gasmara, Cakra
• Format: Journal Article
• Language: English
• Published: University of Brawijaya 01.07.2016
• Subjects: ischemic stroke; ischemic stroke induction method; ischemic stroke mice model; MMP-9; UCAO
• ISSN: 2407-6724; 2442-5001
• DOI: 10.21776/ub.mnj.2016.002.02.1

Background. For the last 5 years, 15.4% of total population died because of stroke, which 42.9% of those are caused by ischemic stroke. UCAO (Unilateral Cerebral Artery Occlusion) is a stroke induction method by ligating mice’s carotid artery for 45 minutes. Thus, giving a hypoxic condition similar to stroke attack in human. This method is less complicated and far more efficient. MMP-9 is a stroke marker which is assayed by ELISA from the blood of test animal. Objective. This research was conducted to prove UCAO (Unilateral Cerebral Artery Occlusion) method is capable to raise MMP-9 concentration in mice’s blood. Methods. This research was an experimental laboratory research with post-test only controlled group design. 8 male rats (8-10 weeks) were divided into 2 groups, control and treatment which would be inducted into stroke by UCAO method. A day after the treatment group had been induced to stroke, both group were tested to measure the MMP-9 blood concentration through ELISA. Results. In this research, UCAO method had increased MMP-9 blood concentration in treatment group, compared to the control group. It is proved by the statistic tests, Mann-Whitney and Kruskal-Wallis, which showed a significant increase in treatment group (p < 0.05). Conclusion. Based on this result, it can be concluded that UCAO method is accepted as a method to create an ischemic stroke mice model.