14C labelling as a reliable technique to screen soybean genotypes (Glycine max (L.) Merr.) for iron deficiency tolerance

• Published in: Journal of radioanalytical and nuclear chemistry Vol. 322; no. 2; pp. 655 - 662
• Main Authors: Raj, Kiran Karthik, Pandey, R. N., Singh, Bhupinder, Talukdar, A.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 2019; Springer Nature B.V
• Subjects: Chemistry; Chemistry and Materials Science; Chlorophyll; Diagnostic Radiology; Exudation; Hadrons; Heavy Ions; Hydroponics; Inorganic Chemistry; Iron; Nuclear Chemistry; Nuclear Physics; Physical Chemistry; Soybeans; Screening; C; Soybean; Root exudates; Iron deficiency chlorosis
• ISSN: 0236-5731; 1588-2780
• DOI: 10.1007/s10967-019-06708-1

Fifty diverse soybean genotypes were screened for their ability to tolerate iron deficiency stress in a hydroponics experiment with low iron (−Fe) and sufficient iron (+Fe). We hypothesised that the genotypes with higher root exudation potential would exhibit higher chlorophyll content, dry matter production and Fe acquisition. The relative root exudation capacity of the genotypes was estimated with the help of 14 C. As compared to iron inefficient and non responsive (FeINR) category under sufficient availability of iron (+Fe), the average 14 C content in the total root exudates ( 14 CTRE) was 39.4% higher in iron efficient and responsive (FeER) category. Further, higher exudation was observed under iron limiting (−Fe) stress condition and reported maximum in FeER (110.0% increase over FeINR under +Fe condition). The strength of positive correlation between 14 C released with other parameters related to iron deficiency chlorosis suggested that 14 C could be effectively used as a tracer for providing reliable estimate for better screening of iron efficient and responsive categories of soybean genotypes.