Determination of 14 Perfluorinated Compounds in Wine by High Performance Liquid Chromatography-Mass Spectrometry

• Published in: Fēnxī huàxué Vol. 41; no. 12; pp. 1893 - 1898
• Main Authors: LAN, Fang, FENG, Sha, SHEN, Jin-Can, WU, Xiao-Ping, YUE, Zhen-Feng
• Format: Journal Article
• Language: Chinese; English
• Published: 01.01.2013
• Subjects: Cartridges; Evaporation; Liquids; Monitoring; Quantitative analysis; Spectrometry; Standard deviation; Wines
• ISSN: 0253-3820
• DOI: 10.3724/SP.J.1096.2013.21133

A LC-MS/MS method had been developed for the determination of 14 perfluorinated compounds (PFCs)in wine. About 3.0 mL wine sample was purified and enriched by WAX cartridge, and then it was eluted with 10 mmol/L triethylamine-methanol solution. The eluent was evaporated under nitrogen and redissolved by methol. The preparation was separated with a Poroshell 120 EC-C sub(18) column and detected by electrospray ionization in negative mode using multiple reaction monitoring (MRM). Internal standard method was used for quantitative analysis. The linear correlation coefficients (r) of all 14 PFCs were more than 0.998 in the concentration range of 0.1-20.0 mu g/L. The limits of detection (LODs) of PFCs were between 0.02 and 0.19 mu g/L, while the limits of quantitation (LOQs) were between 0.16 and 0.63 mu g/L. The recoveries of all PFCs were at 83.0%-117.0%, and the relative standard deviations were less than 15.0% at the spiked levels of 0.5, 1.0 and 5.0 mu g/L. The developed method had been applied to the analysis of several wine samples.