Correlation between expression level of functional genes and tanshinones' accumulation in Salvia miltiorrhiza from different areas

• Published in: Zhongguo zhongyao zazhi Vol. 36; no. 24; p. 3406
• Main Authors: Li, Beining, Zhou, Xiaoli, Huang, Luqi, Wang, Xueyong, Liu, Chunsheng
• Format: Journal Article
• Language: Chinese
• Published: China 15.12.2011
• Subjects: Diterpenes, Abietane - metabolism; Gene Expression; Real-Time Polymerase Chain Reaction; Salvia miltiorrhiza - genetics; Salvia miltiorrhiza - metabolism
• ISSN: 1001-5302
• DOI: 10.4268/cjcmm20112404

To study the relationship between functional gene expression in Salvia miltiorrhiza from different producing areas and active principles, which might provide scientific basis for the gene regulation of tanshinones. The quantitative determination of cryptotanshinone and tanshinone II A was carried out by using HPLC method, expression level of 3 functional genes of SmAACT, SmCMK and SmIPPI were investigated by real-time PCR method. The content of active principles together with expression level of SmAACT and SmCMK were higher in S. miltiorrhiza from genuine producing areas including Henan and Shanxi, but lower in samples from Beijing which was non-genuine producing area. Expression level of SmAACT and SmCMK had close relationships involving tanshinones' accumulation, but the SmIPPI gene had not.