Characterization of novel neopullulanase fromBacillus polymyxa

Bacillus polymyxa CECT 155 produces an extracellular neopullulanase activity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mol wt determined for the enzyme was 58 kDa. The optimum pH and temperature f...

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Published inApplied biochemistry and biotechnology Vol. 68; no. 1-2; pp. 113 - 120
Main Authors Yebra, Maria Jesus, Arroyo, Jaime, Sanz, Pascual, Prieto, Jose Antonio
Format Journal Article
LanguageEnglish
Published Totowa Springer Nature B.V 01.10.1997
Subjects
Bacillus polymyxa
Biophysics
Biotechnology
Enzymes
Neopullulanase
pH effects
Polymyxa
Pullulan
Saccharification
Temperature
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Summary:Bacillus polymyxa CECT 155 produces an extracellular neopullulanase activity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mol wt determined for the enzyme was 58 kDa. The optimum pH and temperature for neopullulanase activity were pH 6.0 and 50°C, respectively. The enzyme was stable in a pH range of 4.0-8.0, and temperatures up to 60°C. These properties make it suitable for the saccharification processes in the starch industries.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0273-2289
1559-0291
DOI:10.1007/BF02785984