Qualitative Indicators of Microbial Cells of Yersinia pestis EV Strain Depending on Their Morphological Traits in Different Temperature Conditions of Manufacturing of Plague Vaccine Preparation

• Published in: Problemy osobo opasnyh infekcij no. 4(114); pp. 33 - 35
• Main Authors: Budyka, D. A., Bondarenko, A. I., Fisun, A. A., Abzaeva, N. V., Ivanova, G. F., Gostishcheva, S. E., Rudnev, S. M.
• Format: Journal Article
• Language: English; Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 20.08.2012
• Subjects: cell morphology; electron microscopy; live plague vaccine; lyophilization; quality parameters of stock-produced preparation; temperature condition; вакцина чумная живая; лиофилизация; морфология клетки; параметры качества готового препарата; температурный режим; электронная микроскопия
• ISSN: 0370-1069; 2658-719X
• DOI: 10.21055/0370-1069-2012-4-33-35

The preference of the (21±1) °C temperature for cultivation of the vaccine strain Yersinia pestis EV in the live plague vaccine biomass production to (27±1) °C is experimentally confirmed. An original interpretation of the mechanism of small cells stability that are formed at (21±1) °C has been proposed. This interpretation stipulates a smaller damaging effect of steam molecules during sublimation in the process of lyophilization.