Nested oscillatory dynamics in cortical organoids model early human brain network development

• Published in: bioRxiv
• Main Authors: Trujillo, Cleber A, Gao, Richard, Negraes, Priscilla D, Chaim, Isaac A, Domissy, Alain, Vandenberghe, Matthieu, Devor, Anna, Yeo, Gene W, Voytek, Bradley, Muotri, Alysson R
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 29.06.2018
• Subjects: Brain; Cortex; EEG; Glutamatergic transmission; Organoids; Oscillations; Transcription; γ-Aminobutyric acid
• DOI: 10.1101/358622

Structural and transcriptional changes during early brain maturation follow fixed developmental programs defined by genetics. However, whether this is true for functional network activity remains unknown, primarily due to experimental inaccessibility of the initial stages of the living human brain. Here, we analyzed cortical organoids that spontaneously developed periodic and regular oscillatory network events that are dependent on glutamatergic and GABAergic signaling. These nested oscillations exhibit cross-frequency coupling, proposed to coordinate neuronal computation and communication. As evidence of potential network maturation, oscillatory activity subsequently transitioned to more spatiotemporally irregular patterns, capturing features observed in preterm human electroencephalography (EEG). These results show that the development of structured network activity in the human neocortex may follow stable genetic programming, even in the absence of external or subcortical inputs. Our model provides novel opportunities for investigating and manipulating the role of network activity in the developing human cortex.