ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

• Published in: Plant breeding and biotechnology pp. 262 - 269
• Main Authors: 손재한, 강천식, 김경훈, 신상현, 김학신, 김남수, 현종내, 심상인, 이춘기, 박광근
• Format: Journal Article
• Language: English
• Published: 한국육종학회 30.09.2013
• Subjects: 농학
• ISSN: 2287-9358; 2287-9366
• DOI: 10.9787/PBB.2013.1.3.262

Inter simple sequence repeat (ISSR) is a technique that leads to the development of novel specific molecular markers and relationship analysis between species. In this study, 54 ISSRs were used to develop cultivar-specific DNA sequence characterized amplified regions (SCARs) for Korean wheat cultivars (Triticum aestivum). Using nine of 54 UBC ISSR primers, 16 cultivar-specific fragments were detected among 32 wheat cultivars. Of the 16 primers, 10 successfully amplified targets in 32 cultivars and six novel cultivar-specific markers were finally selected. A total of 13 Korean wheat cultivars (Ol, Dahong, Namhae, Jinpum, Anbaek, Jopoom,Jokyung, Sinmichal 1, Jeokjoong, Dajung, Goso and Joah) were classified by newly developed six SCAR markers. Cultivar-specific markers can be used not only for cultivar fingerprinting but also for marker-assisted selection in wheat breeding program. KCI Citation Count: 9