Quantitative evaluation of central-type benzodiazepine receptors with [ 125I] Iomazenil in experimental epileptogenesis

This study aimed at quantitatively evaluating hippocampal central-type benzodiazepine receptors (BZRs) in the kainate model of temporal lobe epilepsy (TLE) by in vitro autoradiography (ARG) using [ 125I] Iomazenil (IMZ) specific ligand for central-type BZRs. Kainate (1 μg/0.5 μl) was injected into t...

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Published inEpilepsy research Vol. 61; no. 1; pp. 105 - 112
Main Authors Tamagami, Hiroshi, Morimoto, Kiyoshi, Watanabe, Takemi, Ninomiya, Takashi, Hirao, Toru, Tanaka, Akihiro, Kakumoto, Miki
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.09.2004
Subjects
Benzodiazepine receptor
Central-type
Iomazenil
Kainate model
Rat
Temporal lobe epilepsy
Temporal lobe epilepsy
Kainate model
Benzodiazepine receptor
Central-type
Rat
Iomazenil
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Summary:This study aimed at quantitatively evaluating hippocampal central-type benzodiazepine receptors (BZRs) in the kainate model of temporal lobe epilepsy (TLE) by in vitro autoradiography (ARG) using [ 125I] Iomazenil (IMZ) specific ligand for central-type BZRs. Kainate (1 μg/0.5 μl) was injected into the left amygdala to induce limbic status epilepticus. One, three, or six months after injection, in vitro ARG with [ 125I] IMZ and cell counts were performed in the hippocampal CA1–4 regions and dentate gyrus ipsilateral to the kainate injection site, and were compared with the vehicle-injected control group. In all kainate-treated rats, clear pyramidal neuron loss was observed in left hippocampal areas CA1–4. Compared with the control group, progressive reduction of [ 125I] IMZ binding was also observed. This resulted in a marked binding decrease paralleling pyramidal neuron loss in hippocampal areas CA1 (down to 83% of control), CA2 (76%), CA3 (75%), and CA4 (90%) at 6 months after kainate administration. Conversely, [ 125I] IMZ binding significantly increased in the dentate gyrus (up to 106% of control) at 1 month, but returned to nearly normal at 3–6 months. These results suggest that central-type BZR neuroimaging is useful in detecting hippocampal sclerosis in the mesial TLE, though central BZR alterations differ depending on hippocampal subfields and post-seizure time-courses.
ISSN:0920-1211
1872-6844
DOI:10.1016/j.eplepsyres.2004.07.011