Inflammatory Factors Predicting Dry Eye Syndrome in a Model Using Osmotic Pressure

• Published in: Daihan angwa haghoi jabji Vol. 64; no. 12; pp. 1191 - 1197
• Main Authors: Park, Keon Woo, Koh, Jae Woong
• Format: Journal Article
• Language: English
• Published: 15.12.2023
• ISSN: 0378-6471; 2092-9374
• DOI: 10.3341/jkos.2023.64.12.1191

Purpose: To identify inflammatory factors that may serve as biomarkers for dry eye syndrome using a hyperosmotic dry eye syndrome model.Methods: Cultured human conjunctival epithelial cells were subjected to 400, 450, 500, and 550 mOsm/L NaCl, and cell viability was assessed in response to osmolarity. The relative expression of inflammatory factors was evaluated by real-time polymerase chain reaction of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, metalloproteinase (MMP)-2, and MMP-9 according to the NaCl concentration.Results: An increase in the concentration of NaCl led to a notable decrease in cell viability (p < 0.05). IL-8, TNF-α, and MMP-9 expression levels increased significantly in proportion to NaCl concentration (p < 0.05).Conclusions: IL-8, TNF-α, and MMP-9 may serve as effective biomarkers in dry eye studies.