trans Activation of Gene Expression by v-myb

• Published in: Molecular and cellular biology Vol. 10; no. 5; pp. 2285 - 2293
• Main Authors: Ibanez, Carlos E., Lipsick, Joseph S.
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 01.05.1990
• ISSN: 1098-5549; 1098-5549
• DOI: 10.1128/mcb.10.5.2285-2293.1990

The v-myb oncogene causes acute myelomonocytic leukemia in chickens and transforms avian myeloid cells in vitro. Its product, p48 v- myb , is a short-lived nuclear protein which binds DNA. We demonstrate that p48 v- myb can function as a trans activator of gene expression in transient DNA transfection assays, trans activation requires the highly conserved amino-terminal DNA-binding domain and the less highly conserved carboxyl-terminal domain of p48 v- myb , both of which are required for transformation. Multiple copies of a consensus sequence for DNA binding by p48 v- myb inserted upstream of a herpes simplex virus thymidine kinase promoter are strongly stimulatory for transcriptional activation by a v-myb-VP16 fusion protein but not by p48 v- myb itself, suggesting that the binding of p48 v- myb to DNA may not be sufficient for trans activation.