Interaction of a synthetic peptide of the interferon α‐2 C‐terminal part with human blood leukocytes

• Published in: FEBS letters Vol. 295; no. 1-3; pp. 70 - 72
• Main Authors: Danilkovitch, A.V., Kharitonenkov, A.I., Freze, K.V., Shevalier, A.F., Kolosova, O.V., Bulargina, T.V., Kirkin, A.F., Gusev, M.V.
• Format: Journal Article
• Language: English
• Published: 16.12.1991
• Subjects: a synthetic peptide representing the IFN α-2 amino acid residues 124–138; ConA; concanavalin A; Human; IFN; IFN α-2; interferon; Lymphocyte; PBMC; peptide 2438; peripheral blood mononuclear cells; Specific binding; Synthetic peptide
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(91)81387-N

A biologically active synthetic peptide, 2438, representing the 124–138 amino acid sequence of the human interferon α‐2 (IFN α‐2) molecule, which is known to possess IFN‐like antiproliferative activity, specifically binds to human blood leukocytes. Scatchard plots reveal two different K fd values, for the ‘low’ and ‘high’ affinity binding. The interaction of the 125I‐labelled peptide 2438 with the cells is not impaired by human IFN α‐2 or cholera toxin.