Phenolic compounds profile in extracts of Smilax spp., antioxidant activity, and inhibition of advanced glycation end products

• Published in: Food chemistry Vol. 463; no. Pt 3; p. 141389
• Main Authors: Valle-Sánchez, Sergio Luis, Rodríguez-Ramírez, Roberto, Ávila-Villa, Luz Angélica, Villa-Lerma, Alma Guadalupe, Wall-Medrano, Abraham, de la Rosa, Laura Alejandra, Muñoz-Bernal, Óscar Adrián, González-Córdova, Aarón Fernando, Arellano-Gil, Maritza
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 21.09.2024
• Subjects: AGEs; Antioxidant; HPLC-MS/MS; Polyphenols; ROS; Smilax; Polyphenols; AGEs; HPLC-MS/MS; Antioxidant; Smilax; ROS
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2024.141389

Smilax genus possesses bioactive properties attributed to phenolic compounds, which may exhibit antioxidant effects and inhibit the advanced glycation end products (AGEs). However, identifying these phenolic compounds and AGEs has become increasingly relevant to understanding such activities. This study aimed to identify phenolic compounds in extracts of Smilax spp. and evaluate their antioxidant and AGEs inhibitory activities. To achieve this, the Smilax genus was identified via PCR, and phenolic compounds including chlorogenic acid, naringenin-6-C-glucoside, quercetin, quercetin-3-O-glucoside, and myricetin were identified using HPLC-MS/MS. Antioxidant activity was assessed by ferric reducing antioxidant power (FRAP), and radicals such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis-[3-ethyl-benzothiazoline]-6-sulfonic acid (ABTS), while AGEs inhibition was evaluated using a model system formed by bovine serum albumin-glucose. The highest antioxidant activity was 3612.18 mM TE/g, and the inhibition of AGEs was 52.44 %. These results demonstrate that Smilax spp. can inhibit AGEs, neutralize free radicals, and reduce compounds associated with antioxidant capacity. •Phenolic compounds were identified and quantified by HPLC-MS/MS in extracts from Smilax spp.•Extracts the Smilax spp. have phenolic compounds with antioxidant activity and AGEs inhibitory.•The phenolic compound content is correlated with antioxidant activity but not AGEs inhibition.