Functional analysis of the extraplastidial TRX system in germination and early stages of development of Arabidopsis thaliana

• Published in: Plant science (Limerick) Vol. 350; p. 112310
• Main Authors: Nascimento, Carolina Pereira, da Fonseca-Pereira, Paula, Ferreira-Silva, Marcelle, Rosado-Souza, Laise, Linka, Nicole, Fernie, Alisdair R., Araújo, Wagner L., Nunes-Nesi, Adriano
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.01.2025
• Subjects: Germination; Mitochondria; Redox; Respiration; Seed development; Thioredoxin; Redox; Mitochondria; Thioredoxin; Respiration; Seed development; Germination; germination; seed development; respiration; thioredoxin; mitochondria; redox
• ISSN: 0168-9452; 1873-2259; 1873-2259
• DOI: 10.1016/j.plantsci.2024.112310

A series of processes occur during seed formation, including remarkable metabolic changes that extend from early seed development to seedling establishment. The changes associated with processes initiated mainly after seed imbibition are usually characterized by extensive modification in the redox state of seed storage proteins and of pivotal enzymes for reserve mobilization and usage. Such changes in the redox state are often mediated by thioredoxins (TRXs), oxidoreductase capable of catalyzing the reduction of disulfide bonds in target proteins to regulate its structure and function. Here, we analyzed the previously characterized Arabidopsis mutants of NADPH-dependent TRX reductase types A and B (ntra ntrb), two independent mutant lines of mitochondrial thioredoxin o1 (trxo1) and two thioredoxin h2 (trxh2) mutant lines. Our results indicate that plants deficient in the NADPH dependent thioredoxin system are able to mobilize their reserves, but, at least partly, fail to use these reserves during germination. TRX mutants also show decreased activity of regulatory systems required to maintain redox homeostasis. Moreover, we observed reduced respiration in mutant seeds and seedlings, which in parallel with an impaired energy metabolism affects core biological processes responsible for germination and early development of TRX mutants. Together, these findings suggest that the lack of TRX system induces significant change in the respiration of seeds and seedlings, which undergo metabolic reprogramming to adapt to the new redox state. •Redox regulation by NTRA and NTRB plays a significant role in the respiratory metabolism of seeds and seedlings.•The mobilization of seed reserves is possible even with deficiencies in the NADPH-dependent thioredoxin system.•However, such deficiencies can impact the utilization of these reserves during seed germination.