Inositol Hexaphosphate (IP6): Modulation of Cell Cycle and Proliferation of Bladder Cancer in vitro

Introduction: We hypothesized that inositol hexaphosphate (IP6) would modulate cell cycle and cellular growth in bladder cancer. Materials and Methods: Bladder cancer cell lines were treated with 2.5 or 4.5 mM/well IP6. Cell viability was measured by MTT at 24 and 48 h. Cell cycle analysis was measu...

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Bibliographic Details
Published inCurrent urology Vol. 3; no. 3; pp. 136 - 140
Main Authors Zaslau, Stanley, Riggs, Dale R., Jackson, Barbara J., Talug, Can, Kandzari, Stanley J.
Format Journal Article
LanguageEnglish
Published Basel, Switzerland 01.11.2009
Subjects
Original Paper
Inositol hexaphosphate
Cellular proliferation
Bladder cancer
Cell cycle
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Summary:Introduction: We hypothesized that inositol hexaphosphate (IP6) would modulate cell cycle and cellular growth in bladder cancer. Materials and Methods: Bladder cancer cell lines were treated with 2.5 or 4.5 mM/well IP6. Cell viability was measured by MTT at 24 and 48 h. Cell cycle analysis was measured by DNA staining and quantified by FACS analysis. Results: Cell growth was inhibited in the TCCSUP, HTB9 and T24 cells (p < 0.001) with the exception of the HTB9 cells at 24 h. G1 phase fractions increased in the TCCSUP and HTB9 cells, while S phase fraction was decreased (p < 0.001). G2 phase was decreased in the TCCSUP cells (p < 0.001). G1 phase fraction was decreased in the T24 line with 4.5 mMIP6 (p < 0.001), while a significant increase in the S phase was observed in the T24 cells (p < 0.001). Conclusions: These results indicate that IP6 is potentially a highly effective treatment for carcinoma of the bladder.
ISSN:1661-7649
1661-7657
DOI:10.1159/000253371