Inter- and intraspecific variation between populations of Globodera rostochiensis and G. pallida revealed by random amplified polymorphic DNA

• Published in: Phytopathology Vol. 84; no. 8; pp. 807 - 811
• Main Authors: Folkertsma, R.T. (Wageningen Agricultural University, Wageningen, Netherlands), Rouppe van der Voort, J.N.A.M, Gent-Pelzer, M.P.E. van, Groot, K.E. de, Bos, W.J. van den, Schots, A, Bakker, J, Gommers, F.J
• Format: Journal Article Conference Proceeding
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01.01.1994
• Subjects: ADN; AMPLIFICATION CHAINE POLYMERASE; Animals; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; GLOBODERA PALLIDA; GLOBODERA ROSTOCHIENSIS; INFECCIONES POR NEMATODOS; Invertebrata; Invertebrates; NEMATODOSE; PAISES BAJOS; PATHOTYPE; PATOTIPOS; PAYS BAS; POLIMORFISMO GENETICO; POLYMORPHISME GENETIQUE; Population genetics, reproduction patterns; REACCION DE CADENAS DE POLIMERASA; SOLANUM TUBEROSUM; VARIACION GENETICA; VARIATION GENETIQUE; Natural population; Genetic variability; Genetic marker; Biological evolution; Population genetics; Pest; Phytophagous; Pathotype; Molecular probe; Intraspecific comparison; Nematoda; Nucleic acid; Interspecific comparison; Globodera rostochiensis; Globodera pallida; Polymerase chain reaction; Molecular evolution; DNA; Sibling species; Morphology; Fingerprint method; Helmintha; RAPD markers; Nemathelminthia; Invertebrata
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-84-807

The genetic relationships between populations of the potato cyst nematode species Globodera rostochiensis and G. pallida were analyzed using random amplified polymorphic DNA (RAPD). Only nine of 250 amplified DNA fragments were common to both species. The intraspecific variation was small. The proportions of shared DNA fragments among G. rostochiensis populations ranged from 0.870 to 0.967 and those for G. pallida populations, from 0.829 to 1.000. Unweighted pair group method with arithmetic mean analysis of RAPD data showed that G. rostochiensis populations cluster in groups with similar pathotype designations. No similarity between RAPD data and pathotype classifications of the G. pallida populations was found. The origin of the observed inter- and intraspecific variation and the value of the RAPD technique to determine these variations are discussed