Human Ribosomal DNA Fragments Amplified in Hamster Cells Are Transcribed Only by RNA Polymerase II and Are Not Silver Stained

Cloned human rRNA gene fragments that included the promoter region were introduced into Chinese hamster dihydrofolate reductase-deficient (dhfr - ) cells by cotransformation with a dhfr minigene and amplified by selection for methotrexate resistance. The human ribosomal DNA was transcribed by RNA po...

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Published inMolecular and cellular biology Vol. 7; no. 3; pp. 1289 - 1292
Main Authors Dhar, Veena N., Miller, Dorothy A., Kulkarni, Ashok B., Miller, Orlando J.
Format Journal Article
LanguageEnglish
Published Taylor & Francis 01.03.1987
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Summary:Cloned human rRNA gene fragments that included the promoter region were introduced into Chinese hamster dihydrofolate reductase-deficient (dhfr - ) cells by cotransformation with a dhfr minigene and amplified by selection for methotrexate resistance. The human ribosomal DNA was transcribed by RNA polymerase II, not RNA polymerase I or III. The metaphase chromosome regions containing the transcriptionally active human ribosomal DNA failed to show silver staining.
ISSN:1098-5549
1098-5549
DOI:10.1128/mcb.7.3.1289-1292.1987