Normal development of follicular oocytes collected from prepubertal gilts after gamete intrafallopian transfer

To assess the potential of prepubertal gilts as a source of ova for swine embryo transfer research, (1) oocytes were aspirated from preovulatory follicles following treatment with exogenous gonadotropins and (2) the fertilization and developmental ability of these oocytes was examined by the gamete...

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Published inKachiku hanshokugaku zasshi (Tōkyō. 1977) Vol. 35; no. 4; pp. 217 - 221
Main Authors Chi, C.H. (Nisshin Flour Milling Co. Ltd., Tokyo (Japan)), Nagashima, H, Kato, Y, Mori, Y, Hoshino, K
Format Journal Article
LanguageEnglish
Japanese
Published THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 25.12.1989
Subjects
ANIMAL DONNEUR
CERDAS
DONANTES
DONOR ANIMALS
FECONDATION
FECUNDACION
FERTILIZATION
FOLICULOS OVARICOS
FOLLICLE
FOLLICULE OVARIEN
GAMETE
GAMETES
GAMETOS
GESTACION
GESTATION
GIFT
LECHON
OOCYTE
OVA
OVARIAN FOLLICLES
OVIDUCTE
OVIDUCTOS
OVIDUCTS
OVULE
OVULO
PIG
PIGLETS
PORCELET
PREGNANCY
PREPUBERTY
SOWS
TRUIE
USES
USOS
UTILISATION
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Summary:To assess the potential of prepubertal gilts as a source of ova for swine embryo transfer research, (1) oocytes were aspirated from preovulatory follicles following treatment with exogenous gonadotropins and (2) the fertilization and developmental ability of these oocytes was examined by the gamete intrafallopian transfer (GIFT) method. Four-month-old prepubertal gilts (n=4) were treated with 2000IU of pregnant mare serum gonadotropin (PMSG) and 750 IU of human chorionic gonadotropin (hCG) to induce follicular growth. The numbers of follicles (5-7 mm) developed by this treat-ment were from 22 to 95 per gilt (52±15 SEM). The oocytes were transferred with ejaculated spermatozoa into three recipient gilts, two of which became pregnant and delivered three piglets. This demonstrates a potential method of embryo transfer using prepubertal gilts as donors of ova.
Bibliography:9300359
L53
ISSN:0385-9932
DOI:10.1262/jrd1977.35.217