Time-encoded stimulated Raman scattering microscopy of tumorous human pharynx tissue in the fingerprint region from 1500-1800  cm -1

Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information d...

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Bibliographic Details
Published inOptics letters Vol. 46; no. 14; p. 3456
Main Authors Hakert, Hubertus, Eibl, Matthias, Tillich, Marie, Pries, Ralph, Hüttmann, Gereon, Brinkmann, Ralf, Wollenberg, Barbara, Bruchhage, Karl-Ludwig, Karpf, Sebastian, Huber, Robert
Format Journal Article
LanguageEnglish
Published United States 15.07.2021
Subjects
Humans
Lasers
Microscopy
Nonlinear Optical Microscopy
Pharynx
Spectrum Analysis, Raman
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Summary:Stimulated Raman scattering (SRS) microscopy for biomedical analysis can provide a molecular localization map to infer pathological tissue changes. Compared to spontaneous Raman, SRS achieves much faster imaging speeds at reduced spectral coverage. By targeting spectral features in the information dense fingerprint region, SRS allows fast and reliable imaging. We present time-encoded (TICO) SRS microscopy of unstained head-and-neck biopsies in the fingerprint region with molecular contrast. We combine a Fourier-domain mode-locked (FDML) laser with a master oscillator power amplifier (MOPA) to cover Raman transitions from 1500-1800 . Both lasers are fiber-based and electronically programmable making this fingerprint TICO system robust and reliable. The results of our TICO approach were cross-checked with a spontaneous Raman micro-spectrometer and show good agreement, paving the way toward clinical applications.
ISSN:1539-4794
DOI:10.1364/OL.424726