Phytochemical Profiling and In Vitro Anticancer Activity of Purified Flavonoids of Andrographis glandulosa

• Published in: Planta Medica International Open Vol. 4; no. 1; pp. e24 - e34
• Main Authors: Cherukupalli, Neeraja, Bhumireddy, Sudarshana Reddy, Akella, Subrahmanya Sarma V, Sataniya, Aaysha, Sripadi, Prabhakar, Khareedu, Venkateswara Rao, Vudem, Dashavantha Reddy
• Format: Journal Article
• Language: English
• Published: Stuttgart · New York Georg Thieme Verlag KG 01.02.2017
• Subjects: Original Article; LC-MS; Acanthaceae; NMR-spectroscopy; Andrographis glandulosa; ESI-MS/MS; anticancer activity; chemical profiling
• ISSN: 2509-9264; 2509-6656
• DOI: 10.1055/s-0043-105274

Abstract The genus Andrographis includes diverse species of medicinal importance. The present study for the first time unravels the chemical profiling and medicinal importance of an unexplored species, Andrographis glandulosa . LC-MS analysis of A. glandulosa methanolic extract disclosed it as a rich source of flavonoids, with 8 minor peaks and 2 major peaks ( m/z 285.0749 and 283.0595). NMR and ESI-MS/MS analyses confirmed the major peaks as (R) 2',5-dihydroxy-7-methoxyflavanone and 2',5-dihydroxy-7-methoxyflavone. Purified compounds exhibited antiproliferative activity on 3 cancer cell lines (HeLa, MIA PaCa and U-87) with IC 50 39.81–46.21 µM for flavanone, and 20.84–25.16 µM for flavone. IC 50 values of flavone are on a par with that of the positive control quercetin (21.78–26.82 µM). HeLa cells treated with 117 µM flavanone showed a loss of MMP (~55% of cells) resulting in apoptosis (~51% of cells). Treatment with 80 µM flavone caused a loss of MMP (~88% of cells) and induced apoptosis in ~70% of cells proving its efficacy over flavanone. Purified flavonoids exhibited cytotoxicity by disrupting mitochondrial membrane, caspase 3 activation and apoptosis. Purified flavonoids appear as promising natural products for developing cancer therapeutics.