Biochemical profiling of in-vitro regenerated and conventionally grown Ocimum basilicum var. thyrsiflora plants

• Published in: Vegetos - International journal of plant research Vol. 34; no. 1; pp. 138 - 143
• Main Authors: Tripathi, Anamika, Abbas, N. S., Nigam, Amrita
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.03.2021
• Subjects: Biomedical and Life Sciences; Life Sciences; Plant Anatomy/Development; Plant Breeding/Biotechnology; Plant Physiology; Phenolicterpenes; GC–MS; Monoterpenes; Terpeneesters; Sesquiterpenes
• ISSN: 2229-4473; 2229-4473
• DOI: 10.1007/s42535-020-00172-z

Ocimum is an aromatic plant species with immense medicinal properties. It contains several bioactive compounds that are used in the preparation of synthetic drugs. The study is aimed to compare the volatile components of in-vitro raised plants with the donor mother plant. The in-vitro regenerated plants showed 52 volatile compounds, whereas the mother plant showed 41 compounds only. It was observed that in-vitro regenerants consisted of a higher percentage of volatiles (38.92%) as compared to the mother plant (28.8%). Many similar constituents were identified from both the mother plant and in-vitro raised plants such as linalool, camphor, and δ -terpineol. However, some constituents were obtained from in-vitro raised plants only such as α -cubebene, β -chamigrene, trans-calamenene, cubebol, spathulenol, isogermacrene D, cedrol, isocitronellol, cheloviolene D, cis- β -terpeniol, thujol, γ -amorphene, γ -gurjunene, and γ -elemene. Two diterpenes (neocembrene and cheloviolene D) were also present in the in-vitro raised plants. The current study provides an alternative approach for quick synthesis of bioactive products of medicinal and other commercial use.