Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy
A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, d...
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Published in | EPJ Web of conferences Vol. 287; p. 3009 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
EDP Sciences
2023
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Online Access | Get full text |
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Summary: | A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. A low numerical aperture objective lens coupled to high dioptric power microlenses, implanted in the tissue, can be beneficial for the reduction of optical aberrations. In this context, we fabricated a system of plano-convex microlenses and microscaffolds on a single chip by means of two-photon polymerization), to be used for non-linear imaging of biological specimens. |
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ISSN: | 2100-014X 2100-014X |
DOI: | 10.1051/epjconf/202328703009 |