Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy

A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, d...

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Published inEPJ Web of conferences Vol. 287; p. 3009
Main Authors Kariman, Behjat S., Nardini, Alessandra, Grassi, Marco, Marini, Mario, Conci, Claudio, Bouzin, Margaux, Collini, Maddalena, Raimondi, Manuela T., Chirico, Giuseppe, Osellame, Roberto, Cerullo, Giulio, Martínez Vázquez, Rebeca
Format Journal Article
LanguageEnglish
Published EDP Sciences 2023
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Summary:A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. A low numerical aperture objective lens coupled to high dioptric power microlenses, implanted in the tissue, can be beneficial for the reduction of optical aberrations. In this context, we fabricated a system of plano-convex microlenses and microscaffolds on a single chip by means of two-photon polymerization), to be used for non-linear imaging of biological specimens.
ISSN:2100-014X
2100-014X
DOI:10.1051/epjconf/202328703009