Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantationin Tibial Fractures

The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or enzymatic digestion. Here, we describe an explant c...

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Published inBio-protocol Vol. 11; no. 15; p. e4107
Main Authors Perrin, Simon, Julien, Anais, de Lageneste, Oriane Duchamp, Abou-Khalil, Rana, Colnot, Céline
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.08.2021
Subjects
Biology
Methods
In vivo cell transplantation
Bone regeneration
Periosteum
Skeletal stem/progenitor cell
In vitro differentiation
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Summary:The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or enzymatic digestion. Here, we describe an explant culture method to isolate periosteum-derived stem/progenitor cells for subsequent and analyses. Periosteal cells (PCs) isolated using this protocol express mesenchymal markers, can be expanded , and exhibit high regenerative potential after transplantation at a fracture site, suggesting that this protocol can be employed for PC production to use in new cell-based therapies.
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Contributed equally to this work
ISSN:2331-8325
DOI:10.21769/BIOPROTOC.4107