Nonenzymatic glycosylation of vitreous proteins in vitro and in the streptozotocin-treated diabetic rat

Nonenzymatic glycosylation has been shown to affect collagen elsewhere in the body in diabetic patients. A fluorescent product of nonenzymatic glycosylation of protein was found to accumulate in the vitreous of rats 2 months after diabetogenic doses of streptozotocin. The same product could be demon...

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Bibliographic Details
Published inRetina (Philadelphia, Pa.) Vol. 10; no. 2; p. 153
Main Authors Shires, T K, Faeth, J A, Pulido, J S
Format Journal Article
LanguageEnglish
Published United States 1990
Subjects
Analysis of Variance
Animals
Blood Glucose - metabolism
Collagen - metabolism
Diabetes Mellitus, Experimental - metabolism
Eye Proteins - metabolism
Glucose - metabolism
Glycosylation
In Vitro Techniques
Male
Rats
Spectrometry, Fluorescence
Streptozocin
Vitreous Body - metabolism
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Summary:Nonenzymatic glycosylation has been shown to affect collagen elsewhere in the body in diabetic patients. A fluorescent product of nonenzymatic glycosylation of protein was found to accumulate in the vitreous of rats 2 months after diabetogenic doses of streptozotocin. The same product could be demonstrated in the same time frame after in vitro incubation of glucose with insoluble protein constituents isolated from vitreous. These in vitro and in vivo studies show that nonenzymatic glycosylation of vitreous collagens does occur. Further studies to examine whether nonenzymatic glycosylation affects vitreous collagen function should be performed.
ISSN:0275-004X
DOI:10.1097/00006982-199004000-00013