Assembly of Dynamic Supramolecular Polymers on a DNA Origami Platform

• Published in: Angewandte Chemie Vol. 133; no. 14; pp. 7690 - 7694
• Main Authors: Schill, Jurgen, Rosier, Bas J. H. M., Gumí Audenis, Berta, Magdalena Estirado, Eva, Greef, Tom F. A., Brunsveld, Luc
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 29.03.2021
• Subjects: Assembly; Biological activity; Biomolecules; Chemistry; Deoxyribonucleic acid; DNA; multicomponent assembly; Polymers; self-assembly; Supramolecular polymers; supramolecular synthesis; Topology
• ISSN: 0044-8249; 1521-3757
• DOI: 10.1002/ange.202016244

Biological processes rely on transient interactions that govern assembly of biomolecules into higher order, multi‐component systems. A synthetic platform for the dynamic assembly of multicomponent complexes would provide novel entries to study and modulate the assembly of artificial systems into higher order topologies. Here, we establish a hybrid DNA origami‐based approach as an assembly platform that enables dynamic templating of supramolecular architectures. It entails the site‐selective recruitment of supramolecular polymers to the platform with preservation of the intrinsic dynamics and reversibility of the assembly process. The composition of the supramolecular assembly on the platform can be tuned dynamically, allowing for monomer rearrangement and inclusion of molecular cargo. This work should aid the study of supramolecular structures in their native environment in real‐time and incites new strategies for controlled multicomponent self‐assembly of synthetic building blocks. DNA origami platforms were used to assemble synthetic supramolecular polymers, retaining their dynamic nature with control over monomer composition and loading and dynamic exchange of molecular cargo. The DNA origami‐templated self‐assembly allows for novel strategies for controlled multicomponent self‐assembly and characterization of synthetic supramolecular systems.