Specificity of immunoassays. I. Effect of plasma proteins on the specificity of steroid immunoassay

The theory of the measurement of the specificity of antigen binding by antibodies is reviewed. The specificity of steroid immunoassay has been investigated using a new solid phase radioimmunoassay system. The effects of plasma proteins on the specificity of the immunoassays have been tested. The met...

Full description

Saved in:
Bibliographic Details
Published inEuropean journal of nuclear medicine Vol. 1; no. 1; p. 37
Main Authors Pratt, J J, Koops, W, Woldring, M G, Wiegman, T
Format Journal Article
LanguageEnglish
Published Germany 1976
Subjects
Aldosterone - blood
Cross Reactions
Progesterone - blood
Radioimmunoassay - methods
Serum Albumin
Testosterone - blood
Online AccessGet more information

Cover

More Information
Summary:The theory of the measurement of the specificity of antigen binding by antibodies is reviewed. The specificity of steroid immunoassay has been investigated using a new solid phase radioimmunoassay system. The effects of plasma proteins on the specificity of the immunoassays have been tested. The methods used and the conclusions reached have been tested with homogeneous antibodies in order to simplify the interpretation of the results obtained. A new criterium of antibody homogeneity is proposed. The importance of correcting all results accurately for "non-specific binding" is emphasised. A new method for plotting radioimmunoassay standard curves is presented.
ISSN:0340-6997
DOI:10.1007/BF00253265