Transfection of murine LMTK- cells with purified HLA class I genes. VII. Association of allele- and locus-specific serological reactivities with respectively the first and second domains of the HLA-B7 molecule

The individual contributions of the first two external domains of the HLA-B7 heavy chain to the expression of allele-specific (B7) and locus-specific (B and C) antigenic determinants were investigated using hybrid class I genes. Hybrid genes were constructed in vitro by exon shuffling between the pa...

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Published inImmunogenetics (New York) Vol. 23; no. 4; p. 246
Main Authors Sodoyer, R, Kahn-Perles, B, Strachan, T, Sire, J, Santoni, M J, Layet, C, Ferrier, P, Jordan, B R, Lemonnier, F A
Format Journal Article
LanguageEnglish
Published United States 01.04.1986
Subjects
Animals
Antibodies, Monoclonal
Antibody Specificity
Epitopes
Genes
HLA Antigens - genetics
HLA Antigens - immunology
HLA-B7 Antigen
Humans
L Cells (Cell Line)
Mice
Polymorphism, Genetic
Radioimmunoassay
Transfection
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Summary:The individual contributions of the first two external domains of the HLA-B7 heavy chain to the expression of allele-specific (B7) and locus-specific (B and C) antigenic determinants were investigated using hybrid class I genes. Hybrid genes were constructed in vitro by exon shuffling between the parent genes HLA-B7, HLA-Cw3, HLA-A3, and H-2Kd, and their expression was monitored following transfection into mouse L cells. The results show that most allele-specific antigenic determinants are associated with the first external domain of the B7 heavy chain, whereas all the locus-specific antigenic determinants tested map to the second external domain.
ISSN:0093-7711
DOI:10.1007/BF00373019