Study of Antibacterial Agent Penetration into Gingival Crevices and Antibacterial Effect after Toothbrushing with Liquid Dentifrice

• Published in: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 36; no. 1; pp. 233 - 241
• Main Authors: SANO, Hiroshi, YAMAMOTO, Mizuya, TERAI, Akiko, SUGANUMA, Nobuo, KANEKO, Kenji, KURIYAMA, Sumio
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY OF PERIODONTOLOGY 1994
• Subjects: Gingival crevice; Liquid dentifrice; Triclosan
• ISSN: 0385-0110; 1880-408X
• DOI: 10.2329/perio.36.233

This study was designed to estimate triclosan concentrations and the antibacterial effect of a liquid dentifrice in gingival crevices, in comparison to a conventional dentifrice. The concentration of triclosan penetrating gingival crevices in front teeth was determined by the HPLC method after toothbrushing at molar sites. To estimate the antibacterial effect, samples of gingival crevicular plaque were collected with sterilized paper-points from gingival crevices after toothbrushing for 60 seconds. The antibacterial effect was measured by counting colonies cultivated on blood agar medium (total bacterial count) and CFAT medium (Actinomyces naeslundii and Actinomyces viscosus). As a result, liquid dentifrice facilitated triclosan penetration into gingival crevices to a significant extent as compared with conventional dentifrice after toothbrushing for 10 and 20 seconds (p< 0.05). Both liquid and conventional dentifrice significantly reduced total bacterial counts, (Actinomyces A. naeslundii, A. viscosus) and (A. viscosus, ) in gingival crevices as compared with the control after toothbrushing for 60 seconds (p<0.05, p<0.01). In addition, the liquid dentifrice significantly reduced the tested bacterial counts as campared with the conventional dentifrice (p<0.05). These findings suggest that the use of a liquid dentifrice is effective in keeping gingival crevices clean as compared with a conventional dentifrice.