New Attachment Formation with Monkey Decalcified and Freeze-dried Bone Allografts in Two-walled Bony Defects

• Published in: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 33; no. 3; pp. 651 - 662
• Main Authors: NOGUCHI, Yoshihiro, NISHIMURA, Kazuaki, NAGAISHI, Masaki, SHIGEYAMA, Yoichiro, NISHIGAKI, Mitsuru, YAMAOKA, Akira
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY OF PERIODONTOLOGY 1991
• Subjects: Decalcified and freeze-dried bone allografts; Flap surgery; New bone formation; New cementum formation; Two-walled bony defect
• ISSN: 0385-0110; 1880-408X
• DOI: 10.2329/perio.33.651

This study was performed to examine whether decalcified and freeze-dried bone allografts (DFBA) in two-walled bony defects would promote new cementum formation on roots as well as in three-walled defects. Four adult monkeys were used. Maxillary lateral incisors were extracted to provide room for implantation. After reflection of buccal mucoperiosteal flaps, defects of 3 mm in depth were created in the mesial alveolar bone of maxillary canines, resulting in two-walled defects with a mesial and palatal bony wall. The mesial root surfaces were throughly planed. DFBA were placed into the bony defects but not into those of controls. The left and right teeth were treated in the same way in each monkey. The animals were sacrificed to yield observation periods of 4 and 8 weeks. Each block of canines including the tooth and surrounding bone was processed for light and electron microscopy. Implant particles were resorbed and replaced by the new bone. Progressive cementogenesis occurred on the root surface corresponding to the new bone. The results suggested that DFBA in two-walled bony defects could enhance the new connective tissue attachment including the formation of new cementum.