Development of new gastric ulcer model induced by ischemia-reperfusion and its application to evaluate the efficacy

• Published in: Japanese Journal of Pharmacology Vol. 71; no. suppl.1; p. 202
• Main Authors: Wada, Kouichirou, Kamisaki, Yoshinori, Kitano, Masayuki, Kishimoto, Yosuke, Nakamoto, Kentaro, Itoh, Tadao
• Format: Journal Article
• Language: English; Japanese
• Published: The Japanese Pharmacological Society 1996
• ISSN: 0021-5198; 1347-3506
• DOI: 10.1016/S0021-5198(19)37047-7

We have reported the effects of gastroprotective drugs, radical scavengers and antacid drugs on the gastric mucosal injury induced by ischemia-reperfusion in rats. In this study, we developed the erosion model to the ulcer model and examined to apply this model on the drug evaluation. After the preparation of acute gastric mucosal injury induced by ischemia-reperfusion, rats were awakened. Just, 24 and 36 hours after the ischemia-reperfusion, mainly erosions with falling off the gastric mucosa were observed. Forty-eight, 72 and 96 hours after the preparation, gastric ulcers with the injury of muscularis mucosa were observed in the area of gastric glands. After 6 to 7 days, natural healing process were also observed. To examine the effect of gastroprotective drug in this ulceration model, we administered sucralfate (30mg/kg/day, p.o.) after the preparation of gastric mucosal injury induced by ischemia-reperfusion. Administration of sucralfate showed the significant inhibition on the ulceration at 48 and 72 hours after the preparation of ischemia-reperfusion injury. This model may be useful to examine the mechanism of ulcerogenesis induced by ischemia-reperfusion and to evaluate the effectiveness of gastroprotective drugs, anti-acid drugs and radical scavengers.