Electrically stimulated release of endogenous dopamine and amino acids from rat brain slices under ischemia-like condition

• Published in: Japanese Journal of Pharmacology Vol. 64; no. suppl.1; p. 231
• Main Authors: Umeda, Yuri, Sumi, Takahiko
• Format: Journal Article
• Language: English; Japanese
• Published: The Japanese Pharmacological Society 1994
• ISSN: 0021-5198
• DOI: 10.1016/S0021-5198(19)50557-1

Our previous study showed regional variation of K^+ -stimulated release of endogenous amino acids from rat brain slices exposed to an ischemia-like condition. In the present experiments, we report electrically stimulated release of dopamine and amino acids from rat brain slices (striatum, medial prefrontal cortex, hippocampus) under an ischemia-like condition. Brain slices were superfused with Krebs-Ringer bicarbonate buffer and subsequently stimulated electrically under normal (10mM glucose and 95%O_2 +5%CO_2 ) or ischemia-like conditions (0mM glucose and 95%N_2 +5%CO_2 ). The ischemia-like condition significantly enhanced electrically stimulated release of dopamine and amino acids. Dopamine release received a much stronger influence from the ischemic condition than amino acid release. On the other hand the effects of the ischemic condition on electrically stimulated release of amino acids were very little when compared to those on K^+ -stimulated amino acids release. The physiological significance of these results will be discussed.