Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa

Simple sequence repeat (SSR) molecular markers were used for genetic diversity analysis and population structure of the cultivated Pearl millet in Benin, West Africa. In order to assess the level of genetic diversity, 14 polymorphic SSR markers were used to screen 114 accessions from different agro-...

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Published inAfrican journal of biotechnology Vol. 16; no. 15; pp. 782 - 790
Main Authors Kifouli, ADEOTI, Gustave, DJEDATIN, Ebenezer, EWEDJE, Thierry, BEULE, Sylvain, SANTONI, Alain, RIVAL, Estelle, JALIGOT
Format Journal Article
LanguageEnglish
Published Academic Journals 12.04.2017
Subjects
Agricultural sciences
Life Sciences
Vegetal Biology
pearl millet (plant)
poaceae
diversité génétique
afrique de l'ouest
pennisetum glaucum
cereal
benin
genêtic variation
simple sequence repeat (SSR) markers
genetic variability
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Summary:Simple sequence repeat (SSR) molecular markers were used for genetic diversity analysis and population structure of the cultivated Pearl millet in Benin, West Africa. In order to assess the level of genetic diversity, 14 polymorphic SSR markers were used to screen 114 accessions from different agro-ecological zones in Benin. SSR markers were found to reveal a total of 57 alleles with an average of 4.071 allele per locus. Genetic diversity index varied from 0.099 to 0.633 with an average of 0.405. The average observed heterozygosity was found to reach 0.425. The analysis of molecular variance showed no real differentiation between regions. Only 5% of genetic variation was observed between samples collected from north-eastern and north-western region. A high level of variation (95%) was observed among accessions. Moreover, both principal component analysis (PCA) and the dendrogram obtained from the genetic distance among accessions revealed the absence of any specific structuration of accessions from each region under study. Our results confirmed diversity among cultivated Pearl millet in Benin and such diversity is not clustering according to geographical patterns.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB2017.15898