A life-extension study of high-yield nonmyeloablative bone-marrow transplantation from young into adult mice
Tissue renewal is a phenomenon based on replacement of vanishing cells by progeny of resident or circulated stem cells (SCs). The delivery of stem cells via circulation should result in stem-cell homing and differentiation into a wide variety of tissues and shows promise for therapy of tissue diseas...
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Published in | Cell and tissue biology Vol. 7; no. 2; pp. 136 - 139 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
SP MAIK Nauka/Interperiodica
2013
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Subjects | |
Online Access | Get full text |
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Summary: | Tissue renewal is a phenomenon based on replacement of vanishing cells by progeny of resident or circulated stem cells (SCs). The delivery of stem cells via circulation should result in stem-cell homing and differentiation into a wide variety of tissues and shows promise for therapy of tissue diseases. Here, we studied whether bone-marrow SC transplantation may promote lifespan. For this purpose, we created C57BL/6 chimeric mice by bone-marrow (BM) transplantation from young, 1.5-month-old donors to 21.5-month-old recipient mice of the same C57BL/6 strain. Transplantation was performed by the recently developed new technique of high-yield nonmyeloablative transplantation that allows high levels of chimerism to be obtained due to a very high amount of transplanted cells (1.5 × 10
8
per mouse or 25% of its total BM count). As a result of the modified technique implementation, the mean post-transplantation life (starting at 21.5 months old) of treated mice was 4.9 months versus 3.4 months for untreated mice. The difference of 1.5 months is a 44% extension of mean post-transplantation life. |
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ISSN: | 1990-519X 1990-5203 |
DOI: | 10.1134/S1990519X13020077 |