A life-extension study of high-yield nonmyeloablative bone-marrow transplantation from young into adult mice

Tissue renewal is a phenomenon based on replacement of vanishing cells by progeny of resident or circulated stem cells (SCs). The delivery of stem cells via circulation should result in stem-cell homing and differentiation into a wide variety of tissues and shows promise for therapy of tissue diseas...

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Bibliographic Details
Published inCell and tissue biology Vol. 7; no. 2; pp. 136 - 139
Main Authors Kovina, M. V., Zuev, V. A., Kagarlitskiy, G. O., Khodarovich, Y. M.
Format Journal Article
LanguageEnglish
Published Dordrecht SP MAIK Nauka/Interperiodica 2013
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Summary:Tissue renewal is a phenomenon based on replacement of vanishing cells by progeny of resident or circulated stem cells (SCs). The delivery of stem cells via circulation should result in stem-cell homing and differentiation into a wide variety of tissues and shows promise for therapy of tissue diseases. Here, we studied whether bone-marrow SC transplantation may promote lifespan. For this purpose, we created C57BL/6 chimeric mice by bone-marrow (BM) transplantation from young, 1.5-month-old donors to 21.5-month-old recipient mice of the same C57BL/6 strain. Transplantation was performed by the recently developed new technique of high-yield nonmyeloablative transplantation that allows high levels of chimerism to be obtained due to a very high amount of transplanted cells (1.5 × 10 8 per mouse or 25% of its total BM count). As a result of the modified technique implementation, the mean post-transplantation life (starting at 21.5 months old) of treated mice was 4.9 months versus 3.4 months for untreated mice. The difference of 1.5 months is a 44% extension of mean post-transplantation life.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X13020077