Patchouli Oil Isolation and Identification of Chemical Components Using GC-MS

• Published in: Indonesian journal of chemical research (Online) Vol. 8; no. 2; pp. 108 - 113
• Main Authors: Souhoka, Fensia Analda, Al Aziz, Andi Zulkifli, Nazudin, Nazudin
• Format: Journal Article
• Language: English
• Published: Department of Chemistry, Pattimura University 30.09.2020
• Subjects: GC-MS, isolation, identification, isolation, patchouli oil
• ISSN: 2338-5359; 2614-2627
• DOI: 10.30598//ijcr.2020.8-fas

This research aims to isolate oil from patchouli (Pogostemon cablin Benth) and identify the chemical components of patchouli oil using Gas Chromatography-Mass Spectrometry (GC-MS). Patchouli plant samples were obtained from Welulu Village, Kolaka Regency, Southeast Sulawesi Province. Fresh patchouli leaves are dried in the sun for four days to a constant weight. Patchouli leaves water content is 77.41% (w/w). Dry patchouli leaves are distilled using the water and steam method for two hours. Water and oil distillates are separated using a separating funnel. Patchouli oil obtained was dried using Na2SO4 anhydrous and yield 0.73% (w/w). The results of GC-MS analysis showed that patchouli oil contained 13 chemical components compounds, namely β-patchoulene (4.56%), cedr-8-ene (1.24%), trans-caryophyllene (7.96%), α-guaiene (18.61%), seychellene (5.70%), patchoulene (2.88%), eremophilene (1.33%), azulene (8.74%), delta-guaiene (18.90%), cyclohexanone (1.10%), globulol (1.88%), veridiflorol (4.39%), and alcohol patchouli (22.7%).