IDENTIFICATION OF SOME BIOLOGICALLY ACTIVE COMPOUNDS IN THE HEMOLYMPH OF HONEY-BEE (APIS MELLIFERA CARPATICA) BY CHEMICAL AND CHROMATOGRAPHICAL MEANS

Fresh hemolymph from honey-bee (Apis mellifera carpatica) was freeze-dried. Biologically active compounds, suitable treated previously, were separated by TLC in different solvent systems. Mild alkaline hydrolysis was used for the cleavage of lactones and organic esters, and partial and total acidic...

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Published inEconomics, Management and Financial Markets Vol. 6; no. 1; pp. 1158 - 1160
Main Authors Nicolae-Daniel, Condur, Duică, Florentina, Velicu, Nicolae, Iga, Dumitru Petru, Voinea, Manuel, Tănase, Petruţ, Gîtman, Silvia, Iga, Silvia
Format Journal Article
LanguageEnglish
Published Woodside Addleton Academic Publishers 01.03.2011
Subjects
Analysis
Apis mellifera
Bees
Biochemistry
Chromatography
Dehydrogenases
Dextrose
Economy
Enzymes
Fatty acids
Glucose
Honey
Honeybee
Hydrolysis
Physiological aspects
Solvents
Steroids
Sugars
Visualization
honeybee
hemolymph
TLC
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Summary:Fresh hemolymph from honey-bee (Apis mellifera carpatica) was freeze-dried. Biologically active compounds, suitable treated previously, were separated by TLC in different solvent systems. Mild alkaline hydrolysis was used for the cleavage of lactones and organic esters, and partial and total acidic hydrolysis for other linkages. Reducing power was measured by Fehling method and ninhidrin reagent for amino-group, in connection with coupling reaction. The nuances of color under UV light were also used.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:1842-3191
1938-212X