Subclassification of murine T cells by computerized microphotometric analysis

Splenocytes separated by physical means and classified as T cells bay immunologic tests and computerized microphotometric analysis are differentiated into subgroups by analysis of the distribution patterns of Feulgen-positive nuclear DNA. In like fashion T cells obtained as purified preparations aft...

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Bibliographic Details
Published inCell biophysics Vol. 1; no. 3; p. 243
Main Authors Olson, G B, Bartels, P H, Anderson, R E
Format Journal Article
LanguageEnglish
Published United States 01.09.1979
Subjects
Animals
Computers
Cytological Techniques
DNA - analysis
Female
Male
Mice
Mice, Inbred A
Mice, Inbred C3H
Photometry
T-Lymphocytes - analysis
T-Lymphocytes - classification
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Summary:Splenocytes separated by physical means and classified as T cells bay immunologic tests and computerized microphotometric analysis are differentiated into subgroups by analysis of the distribution patterns of Feulgen-positive nuclear DNA. In like fashion T cells obtained as purified preparations after separation on a nylon column, and accepted as T cells by micromorphometric analysis were subjected to further computerized morphometric analysis of nuclear DNA to form subgroups of cells. In each case, the number and composition of the detected subgroups were consistent. The classification does not appear to reflect any obvious phases of the cell cycle and is not dependent upon the sex and strain of mice from which the cells were obtained.
ISSN:0163-4992
DOI:10.1007/BF02783666