Change of aldolase gene expression during EGF-stimulated primary hepatocyte culture in rats

The concentration of mRNAs of rat aldolase A, B, C, α-fetoprotein, and albumin were examined during EGF-stimulated primary hepatocyte culture in rats. The concentration of aldolase A (oncofetal type) and a-fetoprotein mRNA increased. S1 analysis revealed that among two types (M type and AH type) ald...

Full description

Saved in:
Bibliographic Details
Published inKanzo Vol. 31; no. 1; pp. 64 - 68
Main Authors YAMAMOTO, Kyosuke, SETOGUCHI, Yoichi, MOTOMURA, Mitsuaki, HORI, Katsuji, OZAKI, Iwata, SAKAI, Takahiro
Format Journal Article
LanguageJapanese
Published The Japan Society of Hepatology 1990
Subjects
EGF
Online AccessGet full text
ISSN0451-4203
1881-3593
DOI10.2957/kanzo.31.64

Cover

More Information
Summary:The concentration of mRNAs of rat aldolase A, B, C, α-fetoprotein, and albumin were examined during EGF-stimulated primary hepatocyte culture in rats. The concentration of aldolase A (oncofetal type) and a-fetoprotein mRNA increased. S1 analysis revealed that among two types (M type and AH type) aldolase A mRNA, only AH type (adult rat liver type) was expressed. In contrast, aldolase B (liver type) and albumin dramatically decreased in concentration. These findings indicate that the growth activated and dedifferenciated hepatocyte may produce the aldolase A and C mRNA, however, resting hepatocyte may continue to function as a differentiated cell and produce the aldolase B mRNA.
ISSN:0451-4203
1881-3593
DOI:10.2957/kanzo.31.64