Erratum to “Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists”

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 829; no. 1; pp. 154 - 159
• Main Authors: Gatti, Rosalba, Cappellin, Enrico, Zecchin, Barbara, Antonelli, Giorgia, Spinella, Paolo, Mantero, Franco, De Palo, Elio Franco
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2005
• Subjects: 11β-HSD; Exercise; Solid-phase extraction; 11β-HSD; Solid-phase extraction; Exercise
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2005.11.018

A functional and basic method for the quantitative analysis of urine cortisol ( F) and cortisone ( E) using a solid-phase extraction (SPE) column and HPLC with ultraviolet detection is here described and validated to analyse urine samples. Urine specimens were analysed to study F and E relation and ratio in athletes and healthy sedentary subjects. The F and E concentrations in random urine specimens were significantly higher in the post-exercise versus pre exercise condition in cyclists ( F: 136 ± 93 nmol/l versus 67 ± 50 nmol/l ( p < 0.001); E: 797 ± 400 nmol/l versus 408 ± 252 nmol/l ( p < 0.001)). The F/ E ratio was 0.18 ± 0.11 versus 0.16 ± 0.07, respectively, and a significant difference was only demonstrated comparing sedentary (0.11 ± 0.07) and cyclist individuals at rest ( p < 0.05).