In vivo 31P-NMR spectroscopy and respiration measurements of anaesthetized goby ( Pomatoschistus sp.) pre-exposed to ammonia

The effects of ammonia pre-exposure on high energy-phosphates and the intermediary metabolism of the marine teleost Goby ( Pomatoschistus sp.) were studied by in vivo 31P-NMR with simultaneous respirometry. No significant effect of ammonia pre-exposure was observed on oxygen uptake, muscle intracell...

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Published inComparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 120; no. 3; pp. 469 - 475
Main Authors Grøttum, Jon Arne, Erikson, Ulf, Grasdalen, Hans, Staurnes, Magne
Format Journal Article
LanguageEnglish
Published Elsevier Inc 01.07.1998
Subjects
31P-NMR
Ammonia
Anaesthesia
High energy-phosphate
Intracellular pH
Marine fish
Oxygen consumption
Toxicity
Intracellular pH
31P-NMR
Anaesthesia
Ammonia
Toxicity
High energy-phosphate
Oxygen consumption
Marine fish
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Summary:The effects of ammonia pre-exposure on high energy-phosphates and the intermediary metabolism of the marine teleost Goby ( Pomatoschistus sp.) were studied by in vivo 31P-NMR with simultaneous respirometry. No significant effect of ammonia pre-exposure was observed on oxygen uptake, muscle intracellular pH or high-energy phosphate metabolism. Brief metomidate anaesthesia (10 mg l −1) followed by continuous light anaesthesia (1 mg l −1) was used to prevent excessive swimming. This had no effect on oxygen uptake, muscle intracellular pH or high-energy phosphate metabolism.
ISSN:1095-6433
1531-4332
DOI:10.1016/S1095-6433(98)10047-8