Substitution of Asn for Ser 543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules

• Published in: FEBS letters Vol. 425; no. 2; pp. 249 - 250
• Main Authors: Ignatov, K.B, Miroshnikov, A.I, Kramarov, V.M
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 27.03.1998
• Subjects: Klentaq DNA polymerase; Polymerase chain reaction; Taq DNA polymerase; Tth DNA polymerase; Polymerase chain reaction; PCR, polymerase chain reaction; Klentaq DNA polymerase; S543N, mutation Ser 543 to Asn; Tth DNA polymerase; Taq DNA polymerase
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(98)00241-5

Substitution of Asn for Ser 543 in the large fragment of Taq DNA polymerase ( Klentaq) increases several times the efficiency of synthesis of long (over 2 kbp) DNA molecules. The difference in the DNA synthesis efficiencies by the mutant and native enzymes increased with the increase in the DNA fragment length.